Processing Pisum sativum seed storage protein precursors in vitro

编号 zgly0000058233

文献类型 期刊论文

文献题名 Processing Pisum sativum seed storage protein precursors in vitro

作者 YANGLIJUN  CDOMONEY 

作者单位 ShanghaiInstituteofPlantPhysiology  AcademiaSinica  Shanghai200032  China 

母体文献 Cell Research;细胞研究: 英文版 

年卷期 1990,1(2)

页码 153-162

年份 1990 

分类号 S330.2  Q945.65 

关键词 豌豆  种子处理  贮藏蛋白前体  豌豆球蛋白 

文摘内容 The profile of polypeptides separated by SDS-PAGE from seed of major crop species such as pea(Pisum sativum) is complex,resulting from cleavage (processing) of precursors expressed from multiple copies of genes encoding vicilin and legumin,the major storage globulins.Translation in vitro of mRNAs hybridselected from mid-maturation pea seed RNAs by defined vicilin and legumin cDNA clones provided precursor molecules that were cleaved in vitro by a cell-free protease extract obtained from similar stage seed;the derived polypeptides were of comparable sizes to those observed in vivo.The feasibility of transcribing mRNA in vitro from a cDNA clone and cleavage in vitro of the derived translation products was established for a legumin clone,providing a method for determining polypeptide products of an expressed sequence.This approach will also be useful for characterising cleavage site requirements since modifications an readily be introduced at the DNA level。