pH Dependence of Chlorophyll States, Protein Structures and Function of the PSII Membranes

编号 zgly0000311502

文献类型 期刊论文

文献题名 pH Dependence of Chlorophyll States, Protein Structures and Function of the PSII Membranes

学科分类 220.1050;树木生理学

作者 李冬海  阮翔  许强  王可玢  公衍道  匡廷云  张秀芳  赵南明 

作者单位 StateKeyLaboratoryofBiomembraneandMembraneBioteehnology  DepartmentofBiologicalSciencesandBioteehnolo 

母体文献 Tsinghua Science and Technology;清华大学学报: 自然科学英文版 

年卷期 2003,8(4)

页码 493-497

年份 2003 

分类号 Q945.11 

关键词 植物生理学  光合体系II  叶绿素  蛋白质  pH值  荧光光谱学 

文摘内容 The effect of varying pH on the photosystem II (PSII) membrane was studied using absorption and steady-state fluorescence spectroscopy, and using a variable fluorescence technique, pH variations induced significant changes in the chlorophyll states of the PSII membrane, but no effect was seen on the chlorophyll fluorescence parameter Fv′/Fm′. For acidic pH conditions, protein structures of the PSII membrane were.slightly altered, whilst at alkaline pH levels, large changes in the protein structure of the PSII membrane were detected. The results indicate that the microenvironment around Cys in the PSII membrane is very susceptible to alkaline pH conditions, and that in the acid (4≤pH<7) and alkaline (9≥pH>7) regions, pH variation has no effect on the protein structures of the PSII reaction center (RC)。