Cloning and Sequence Analysis of a Glucose-6-Phosphate Dehydrogenase Gene PsG6PDH from Freezing-tolerant Populus suaveolens

编号 zgly0000362554

文献类型 期刊论文

文献题名 Cloning and Sequence Analysis of a Glucose-6-Phosphate Dehydrogenase Gene PsG6PDH from Freezing-tolerant Populus suaveolens

学科分类 220.99;林学其他学科

作者 LinYuan-zhen  LinShan-zhi  ZhangWei  ZhangQian  ZhangZhi-yi  GuoHuan  LiuWen-feng 

作者单位 KeyLaboratoryforGeneticsandBreedinginForestTreesandOmarnentalPlants  MinistryofEducation  BeijingForestryUniversity  Beijing100083  P.R.China 

母体文献 Forestry Studies in China;中国林学: 英文版 

年卷期 2005,7(1)

页码 1-6

年份 2005 

分类号 S792.11 

关键词 葡萄糖-6-磷酸脱氢酶  PsG6PDH基因  基因克隆  序列分析  杨  耐寒性 

文摘内容 A 1207 bp cDNA fragment (PsG6PDH) was amplified by RT-PCR from cold-induced total RNA of the freezing-tolerant P. Suaveolens, using primers based on the highly conserved region of published plant glucose-6-phosphate dehydrogenase (G6PDH) genes. The sequence analysis showed that PsG6PDH coding region had 1 101 bp and encoded 367 predicted amino acid residues. Moreover, the nucleotide sequence of PsG6PDH showed 83%, 82%, 79%, 79% and 78% identity, and the derived amino acid sequence shared 44.2%, 44.7%, 42.0%, 40.5% and 43.9% identity with those of the Solanum tuberosum, Nicotiana tabacum, Triticum aestivum, Oryza sativa and Arabidopsis thaliana, respectively. The results show that PsG6PDH is a new member of G6PDH gene family and belongs to the cytosolic G6PDH gene. This is the first report on cloning of the G6PDH gene from woody plants。