cDNA Fragment Cloning of L-Galactono-1,4-Lactone Dehydrogenase andIt＇s Expression in Different Organs of R. roxburghii Tratt

编号 zgly0000345085

文献类型 期刊论文

文献题名 cDNA Fragment Cloning of L-Galactono-1,4-Lactone Dehydrogenase andIt＇s Expression in Different Organs of R. roxburghii Tratt

作者 ANHua-ming  CHENLi-geng  FANWei-guo 

作者单位 DepartmentofHorticulture  ResearchInstituteforFruitResourcesofKarstMountainRegion 

母体文献 Agricultural Sciences in China;中国农业科学: 英文版 

年卷期 2004,3(11)

页码 807-811

年份 2004 

分类号 Q949.751.8  Q943.2 

关键词 维生素C  抗坏血酸  农产品  生理功能  基因表达  蔷薇属  克隆 

文摘内容 A 855 bp cDNA encoding L-galactono-l,4-1actone dehydrogenase (GalLDH) fragment wascloned from fruit of R. roxburghii Tratt by the method of RT-PCR, on the basis of thehomologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc. inGenBank. Sequence analysis showed 79-92% identity in nucleotide sequence and 75-87%identity in amino acid sequence to that of strawberry and Arabidopsis thaliana, etc.Northern blot showed that the expression of GalLDH was significantly different indifferent organs. The transcription level of GalLDH in fruit was significantly higherthan that in leaf, stem and root respectively. Furthermore, this expression mode washighly correlated with AsA levels。