生防因子螺旋毛壳ND35的细胞壁降解酶与重寄生作用

编号 zgly0000379674

文献类型 期刊论文

文献题名 生防因子螺旋毛壳ND35的细胞壁降解酶与重寄生作用

作者 高克祥  刘晓光  DailaFriesem  时呈奎 

作者单位 西北农林科技大学植物保护学院  山东农业大学植物保护学院  希伯莱大学农业食品与环境科学院植病和微生物系及农业生物技术中心 

母体文献 林业科学 

年卷期 2005,41(1)

页码 205-210

年份 2005 

分类号 S476  S435.131 

关键词 细胞壁降解酶  重寄生  IEF  PAGE  因子  螺旋  SEM 

文摘内容 Some Chaetomium spp. are capable of antagonizing several plant pathogenic fungi through production of antibiotics and mycoparasitism. Secretion of lyric enzymes, mainly including glucanases and chitinases, is considered the most important step in the mycoparasitic process. In this study, an about 110kDa exo -β- 1,3 - glucanase from C. spirale ND35 was detected both in culture filtrate and directly on PAGE and IEF gels, as well as chitinases, although protease was not detectable on Litmus milk agar plates. Coiling and penetrating the hyphae of host fungus Valsa mali were observed by scanning electron microscope (SEM), which may be related to the synergistic interaction between β- 1,3 - glucanase and chitinases.β- 1,3 - glucanase activity of C. spirale ND35 varied considerably when C. spirale ND35 was grown in different carbon sources during various incubation time, and might be subjected to both induction by substrate and catabolite repression。